Non-coding RNAs have emerged as crucial regulators of gene expression, epigenetics and cell fate decisions. To better define their role in hematopoiesis, we have profiled miRNA, lncRNA and mRNA expression in human hematopoietic stem cells and their differentiated progeny, including all the mature blood lineages. These ncRNA landscapes were compared with those of 46 pediatric AML patient samples, amounting to more than 40,000 lncRNAs, 25,000 mRNAs and 900 miRNAs quantified on 146 arrays.
Via the lncScape online app, the expression levels of manifold miRNAs and lncRNAs can be retrieved across normal and malignant hematopoiesis. Our resource includes lineage-specific fingerprint lncRNAs, gene set enrichment analyses, and class prediction of co-expressed coding genes, so that users can infer functions for every transcript.